Timeline

2023

mScarlet3 Displays Record Brightness

Reference: Gadella, T.W.J., van Weeren, L., Stouthamer, J. et al. mScarlet3: a brilliant and fast-maturing red fluorescent protein. Nat Methods 20, 541–545 (2023). https://doi.org/10.1038/s41592-023-01809-y

2023

Calcium Detectors Version 8

Reference: Zhang, Y., Rózsa, M., Liang, Y. et al. Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature 615, 884–891 (2023). https://doi.org/10.1038/s41586-023-05828-9

2016

mScarlet: First Monomeric Red Fluorescent Protein

Reference: Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074

2014

GFP Receives Its Second Nobel Prize in Chemistry

Reference: https://www.nytimes.com/2014/10/09/science/nobel-prize-chemistry.html

2011

GFP used as “living” laser for first time.

Reference: MC Gather, Sh Yun: Single-cell biological lasers, Nature Photonics, 2011/06/12/online, advance online publication.

2008

GFP Earns Its First Nobel Prize in Chemistry

Reference: https://www.nobelprize.org/prizes/chemistry/2008/press-release/

2008

The first mutant of the Aequorea victoria GFP that forms a red chromophore reported.

Reference: AS Mishin, FV Subach, IV Yampolsky, W King, KA Lukyanov, VV Verkhusha: The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore. Biochemistry 47 (2008) 4666-73.

2007

FP found in amphioxus, first time in chordates.

DD Deheyn, K Kubokawa, JK McCarthy, A Murakami, M Porrachia, GW Rouse, ND Holland: Endogenous green fluorescent protein (GFP) in amphioxus. Biological Bulletin 213 (2007) 95-100.

2007

mKate, bright far-red FP.

Reference: D Shcherbo, EM Merzlyak, TV Chepurnykh, AF Fradkov, GV Ermakova, EA Solovieva, KA Lukyanov, EA Bogdanova, AG Zaraisky, S Lukyanov, DM Chudakov: Bright far-red fluorescent protein for whole-body imaging. Nature Methods 4 (2007) 741-46.

2007

Molecular thermometer based on FP blinking.

Reference: FHC Wong, DS Banks, A Abu-Arish, C Fradin: A molecular thermometer based on fluorescent protein blinking. Journal of the American Chemical Society 129 (2007) 10302.