FP found in amphioxus, first time in chordates.
DD Deheyn, K Kubokawa, JK McCarthy, A Murakami, M Porrachia, GW Rouse, ND Holland: Endogenous green fluorescent protein (GFP) in amphioxus. Biological Bulletin 213 (2007) 95-100.
mKate, bright far-red FP.
Reference: D Shcherbo, EM Merzlyak, TV Chepurnykh, AF Fradkov, GV Ermakova, EA Solovieva, KA Lukyanov, EA Bogdanova, AG Zaraisky, S Lukyanov, DM Chudakov: Bright far-red fluorescent protein for whole-body imaging.…
New “fruit” FPs generated by in vitro and in vivo directed evolution.
NC Shaner, RE Campbell, PA Steinbach, BNG Giepmans, AE Palmer, RY Tsien: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nature Biotechnology 22…
Kindling protein (KFP) can undergo irreversible photoconversion from non-fluorescent to stable red fluorescent form upon photoactivation.
Reference: DM Chudakov, VV Belousov, AG Zaraisky, VV Novoselov, DB Staroverov, DB Zorov, S Lukyanov, KA Lukyanov: Kindling fluorescent proteins for precise in vivo photolabeling. Nature Biotechnology 21 (2003) 191-94.
Monomeric DsRed (mRFP), first photoconverible (Kaede), and photoactivatable (PA-GFP ) FPs created.
References: RE Campbell, O Tour, AE Palmer, PA Steinbach, GS Baird, DA Zacharias, RY Tsien: A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United…
The biggest difference between green fluorescent protein and its red analog, DsRed, is that the chromophore of DsRed has an extra double bond (drawn in yellow) which extends the chromophores conjugation and causes the red-shift.
Reference: RE Campbell, O Tour, AE Palmer, PA Steinbach, GS Baird, DA Zacharias, RY Tsien: A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United…
Fluorescent timer protein
Reference: LA Gross, GS Baird, RC Hoffman, KK Baldridge, RY Tsien: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proceedings of the National Academy of…
Red fluorescent proteins (DsRed) discovered in anthozoan corals. (19) Leads to discovery of many new fluorescent proteins and chromoproteins. GFP tolerates insertion of entire proteins in certain locations and can be circularly permuted.
Reference: MV Matz, AF Fradkov, YA Labas, AP Savitisky, AG Zaraisky, ML Markelov, SA Lukyanov: Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotech. 17 (1999) 969-73.
Photoisomerization between A(neutral) and B(anionic) forms of wild-type GFP elucidated.
Resource: K Brejc, TK Sixma, PA Kitts, SR Kain, RY Tsien, M Ormo, SJ Remington: Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proceedings…
FRET between two fluorescent proteins linked by the calmodulin binding domain used as a Ca2+ sensor named Cameleon.
References: A Miyawaki, J Llopis, R Heim, JM McCaffrey, JA Adams, M Ikura, RY Tsien: Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388 (1997) 882-87.…
